Hydroxyproline Assay Kit

Catalog # 6017

INTRODUCTION

Collagen is the major structural protein of the extracellular matrix in many tissues. Hydroxyproline, a major component of collagen, makes up about 13.5% of its amino acid composition. Due to its highly restricted distribution in collagen, the hydroxyproline content accurately reflects the amount of collagen. Therefore, quantitating hydroxyproline has been utilized for evaluating tissue fibrosis or collagen deposition (1 - 3).

However, conventional hydroxyproline assays are not useful because they require cumbersome procedures and special tools. Chondrex, Inc. offers a hydroxyproline assay kit (catalog # 6017) which employs an improved assay system that can be operated with ease and precision using 96-well plates.

This kit can quantify the total collagen content in any tissue specimen or tissue homogenate, regardless of collagen type or species. Chondrex,Inc. also provides three additional collagen detection kits for different purposes. Firstly, the Sirius Red Total Collagen Detection Kit (catalog# 9062) can be used to quantify solubilized collagen in samples. Secondly, the collagen detection ELISA kits specific to type I or II collagenof various species (catalog # 6012-6016, 6019, 6018, and 6021) are used for distinguishing collagen types and species in samples. Lastly,the Sirius Red/Fast Green Collagen Staining Kit (catalog # 9046) is a semi-quantitative assay to determine the total collagen content in tissue sections.